Dystroglycan anticorps

N° du produit ABIN1107039

Cet anticorps anti-Dystroglycan est un anticorps Souris Monoclonal détectant Dystroglycan dans WB, IHC (p) et EIA. Adapté pour Humain, Souris, Rat, Boeuf (Vache) et Lapin.

Aperçu rapide pour Dystroglycan anticorps (ABIN1107039)

Antigène: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dystroglycan est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 2238

Détail du produit anti-Dystroglycan anticorps

Specificité: This antibody is specific for a glycoepitope on brain bovine alpha-dystroglycan, which is absent on alpha-dystroglycan expressed in all other tissues.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse, rat, rabbit, bovine

Purification: Protein G purified

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur Dystroglycan

Antigène: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Autre désignation: Dystroglycan

Sujet: Alpha-dystroglycan (alpha-DG), also known as dystrophin-associated glycoprotein, is a laminin-binding protein of ~156 kDa (including glyco-groups). Alpha-DG is a component of the dystroglycan complex, which is involved in early development, morphogenesis and in the pathogenesis of muscular dystrophies. Alpha- and beta-DG are encoded by a single gene and are derived from a precursor polypeptide by posttranslational cleavage. Beta-DG is an integral membrane protein, whereas alpha-DG is membrane-associated through its noncovalent interaction with the extracellular domain of beta-DG. The alpha- and beta-DGs provide important physical linkages between components of basement membranes and cytoplasmic proteins that bind to the actin cytoskeleton. Alpha-DG is a heavily glycosylated, mucin-like protein anchored on the extracellular surface of the myotube, where it may provide linkage between the sarcolemma and extracellular matrix (ECM). Alpha-DG is expressed in a variety of fetal and adult tissues. Tissue-specific glycosylation modifies the laminin specificity of alpha-DG. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alpha-DG has been shown to colocalize with laminin in skeletal and cardiac muscle and a number of other cells including peripheral nerve, astrocytes, Purkinje neurons and kidney epithelium. Laminin-10/11 was shown to bind preferentially to brain alpha-DG. In Duchenne muscular dystrophy, the expression of alpha-DG is dramatically reduced leading to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. In the central nervous system, dystroglycan functions as a dual receptor for agrin and laminin-2 for instance in the Schwann cell membrane. Furthermore, defects in dystroglycan are central to the pathogenesis of structural and functional brain abnormalities seen in congenital muscular dystrophies (CMD).Synonyms: DAG1, Dystrophin-associated glycoprotein 1

ID gène: 281439

NCBI Accession: NP_776587

UniProt: O18738

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus